SLAM-Drop-seq



Experimetally, we applied 4sU metabolic RNA labeling and alkylation to UMI-based single cell RNA sequencing. Computationally, we quantified newly sythesized RNAs from pre-existing ones and classified precursor and muture mRNAs. We futher computationally sorted the sinlge cells along the cell cycle and estimated the gene-specific transcription, splicing and degradation rates over the cell cycle time.



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Features

  • Gene quantification

  • Gene expression and kinetics rates



Release date: 2021-01-07



Expression & rates